PNAS Early Edition contains papers published daily online.When papers appear in an issue, they will be removed from this feature.Structural genomics involves taking a large number of approaches to structure determination, including experimental methods using genomic sequences or modeling-based approaches based on sequence or structural homology to a protein of known structure or based on chemical and physical principles for a protein with no homology to any known structure.As opposed to traditional structural biology, the determination of a protein structure through a structural genomics effort often (but not always) comes before anything is known regarding the protein function.
This is an advantage in probing the role of local entropic contributions to binding using relaxation methods, and in the dissection of localized weak contributions, as in the 'SAR (structure activity relationships) by NMR' approach. The amino acid sequence showed approximately 94% homology with that of bovine heart.7622059] [Full Text]" pmid="7622059"Leckschat et al. (1993) used a partial human c DNA clone corresponding to the iron protein subunit of succinate dehydrogenase in Southern analyses of restriction enzyme digests of genomic human and hamster DNA, as well as hamster-human hybrids containing a limited number of human chromosomes, to demonstrate that the gene is located on human chromosome 1.Using the same genomic clone, they subregionalized the gene to 1p36.1-p35 by fluorescence in situ hybridization. (1980) described an SDH-deficient hamster cell line that was complemented by human chromosome 1.It was presumed that, because it mapped to chromosome 1, the iron sulfur protein subunit gene complemented the deficiency in the mutant. (2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase (FH; 136850) and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer (HLRCC; 150800) and to paragangliomas, respectively.